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Ly6g antibody (Lymphocyte Antigen 6 Complex, Locus G) (FITC) Primary Antibody

Ly6g Reactivity: Mouse FACS, Func, IHC (f), IHC (p), IP, WB Host: Rat Monoclonal RB6-8C5
Pubmed (8)
Catalog No. ABIN457353
$327.89
Plus shipping costs $45.00
0.1 mg
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  • Target
    Reactivity
    Mouse
    Host
    Rat
    Clonality
    Monoclonal
    Conjugate
    This Ly6g antibody is conjugated to FITC
    Application
    Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
    Specificity
    The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.
    Cross-Reactivity (Details)
    Mouse
    Immunogen
    Murine granulocytes
    Clone
    RB6-8C5
    Isotype
    IgG2b
  • Application Notes
    The reagent is designed for Flow Cytometry analysis.Suggested working concentration is 1 µ,g/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
    Comment

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
  • Jung, Zindl, Lai, Weaver, Chaplin: "MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways." in: European journal of immunology, Vol. 39, Issue 8, pp. 2281-92, 2009 (PubMed).

    dos Santos, Vaz Cardoso, Nascimento, Lino, Dorta, de Oliveira, Ribeiro-Dias: "Leishmania major: recruitment of Gr-1+ cells into draining lymph nodes during infection is important for early IL-12 and IFN gamma production." in: Experimental parasitology, Vol. 119, Issue 3, pp. 403-10, 2008 (PubMed).

    Leendertse, Willems, Giebelen, Roelofs, Bonten, van der Poll: "Neutrophils are essential for rapid clearance of Enterococcus faecium in mice." in: Infection and immunity, Vol. 77, Issue 1, pp. 485-91, 2008 (PubMed).

    Fan, Li, Levy, Fan, Hackam, Vodovotz, Yang, Tracey, Billiar, Wilson: "Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 10, pp. 6573-80, 2007 (PubMed).

    Koo, Gan: "The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection." in: BMC immunology, Vol. 7, pp. 19, 2006 (PubMed).

    Nagendra, Schlueter: "Absence of cross-reactivity between murine Ly-6C and Ly-6G." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 58, Issue 2, pp. 195-200, 2004 (PubMed).

    Brown, Blaho, Loiacono: "Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 893-901, 2003 (PubMed).

    Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, 1991 (PubMed).

  • Target
    Alternative Name
    Ly6G
    Synonyms
    Gr-1, Gr1, Ly-6G, lymphocyte antigen 6 complex, locus G, Ly6g
    Background
    Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.
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