Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) antibody Primary Antibody
CXCL1 Reactivity: Human ELISA, Neut, WB Host: Rabbit Polyclonal
Catalog No. ABIN464980
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- ELISA, Neutralization (Neut), Western Blotting (WB)
- Human GRO/MGSA
- Immunoaffinity purified
- E.coli derived recombinant Human GRO/MGSA.
Type of Immunogen: Cell extract
- Application Notes
- Approved: ELISA (0.5 - 2 μg/mL), Neut (1.5 - 3 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 μg/mL-0.150 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human GRO/MGSA (100 ng/mL), a concentration of 1.5-3 μg/mL of this antibody is required. ELISA: To detect Human GRO/MGSA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GRO/MGSA (LS-C104350) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human GRO/MGSA. Western Blot: To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Target Species of Antibody: Human
- For Research Use only
- Sterile water
- Lot specific
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- 4 °C,-20 °C
- Storage Comment
- Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
- Alternative Name
- GRO / CXCL1 (CXCL1 Antibody Abstract)
- Name/Gene ID: CXCL1
Subfamily: Intercrine alpha
Synonyms: CXCL1, C-X-C motif chemokine 1, Fibroblast secretory protein, FSP, GRO1, GROa, Growth-regulated alpha protein, GRO-alpha(1-73), MGSA, MGSA-a, Small inducible cytokine b1, GRO, MGSA alpha, NAP-3, SCYB1
- Gene ID
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