CXCL1 antibody (Biotin)

Catalog No. ABIN465792

The Rabbit Polyclonal anti-CXCL1 antibody is suitable to detect CXCL1 in samples from Rat. It has been validated for WB, ELISA and Neut.

Quick Overview for CXCL1 antibody (Biotin) (ABIN465792)

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CXCL1 Antibody (Biotin)

Specificity: Rat GRO/KC

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Rat GRO/KC.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat GRO/KC by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat GRO/KC. To detect Rat GRO/KC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat GRO/KC (LS-C104778) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat GRO/KC. Western Blot: To detect Rat GRO/KC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat GRO/KC is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CXCL1

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: GRO / CXCL1

Background: Name/Gene ID: CXCL1
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL1, C-X-C motif chemokine 1, Fibroblast secretory protein, FSP, GRO1, GROa, Growth-regulated alpha protein, GRO-alpha(1-73), MGSA, MGSA-a, Small inducible cytokine b1, GRO, MGSA alpha, NAP-3, SCYB1

Gene ID: 2919

UniProt: P09341

Pathways: Autophagy