Filaggrin antibody (AA 998-1104)

Catalog No. ABIN4949675

Product Details anti-Filaggrin Antibody

Target: Filaggrin (FLG)

Binding Specificity: AA 998-1104

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Filaggrin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Amino acids 998-1104 of human FLG were used as the immunogen for this Filaggrin antibody.

Clone: FLG-1561

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Filaggrin antibody should be determined by the researcher.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Filaggrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Filaggrin

Target: Filaggrin (FLG)

Alternative Name: Filaggrin (FLG Products)

Background: Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Pathways: Sensory Perception of Sound, Stem Cell Maintenance