NADPH Oxidase 4 antibody

Catalog No. ABIN4949834

Product Details anti-NADPH Oxidase 4 Antibody

Target: NADPH Oxidase 4 (NOX4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NADPH Oxidase 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purification: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for this NOX4 antibody.

Clone: NOX4-1245

Isotype: IgG2b kappa

Application Details

Application Notes: Titering of the NOX4 antibody may be required for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,ELISA: suitable, order BSA-free formulation for coating,WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the NOX4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Alternative Name: NOX4 (NOX4 Products)

Background: The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.

Gene ID: 4821

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration