DcR2 antibody (Intracellular)

Catalog No. ABIN499724

Product Details anti-DcR2 Antibody

Target: DcR2 (TNFRSF10D) (Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D))

Binding Specificity: AA 249-263, Intracellular

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DcR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: DcR2 antibody was raised against a peptide corresponding to amino acids 249 to 263 of Human DcR2 precursor.

Purification: Affinity Chromatography purified via peptide column

Immunogen: Human DcR2 (Intracellular Domain) Peptide corresponding to amino acids 249 to 263 of Human DcR2 precursor.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1/1000 to 1/2000. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

References for anti-DcR2 antibody (ABIN499724)

Liu, Hawkins, Vilgelm, Pawlikowski, Ecsedy, Sosman, Kelley, Richmond: "Combining an Aurora Kinase Inhibitor and a Death Receptor Ligand/Agonist Antibody Triggers Apoptosis in Melanoma Cells and Prevents Tumor Growth in Preclinical Mouse Models." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 21, Issue 23, pp. 5338-48, (2015) (PubMed).

Target Details for DcR2

Target: DcR2 (TNFRSF10D) (Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy with Truncated Death Domain (TNFRSF10D))

Alternative Name: CD264 / TRAILR4 (TNFRSF10D Products)

Background: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL, and DcR1/TRID is a decoy receptor (1-3). Another member of the TRAIL receptor family was more recently identified and designated DcR2, TRAIL-R4, or TRUNDD (4-6). DcR2 has an extracellular TRAIL-binding domain but lacks intracellular death domain and does not induce apoptosis. Like DR4 and DR5, DcR2 transcript is widely expressed in normal human tissues. Overexpression of DcR2 attenuated TRAIL-induced apoptosis.Synonyms: DCR2, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TNFRSF10D, TRAIL receptor 4, TRAIL receptor with a truncated death domain, TRAIL-R4, TRUNDD, Tumor necrosis factor receptor superfamily member 10D

Gene ID: 8793

UniProt: Q9UBN6

Pathways: Apoptosis