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KRT18 antibody (Keratin 18) (AA 204-430) Primary Antibody

KRT18 Reactivity: Human, Mouse, Rat IHC (p), WB Host: Rabbit Polyclonal
Pubmed (6)
Catalog No. ABIN5518769
$280.00
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100 μg
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  • Target
    Binding Specificity
    AA 204-430
    Reactivity
    Human, Mouse, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This KRT18 antibody is un-conjugated
    Application
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 18(KRT18) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 18(KRT18) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: keratin 18
    Protein Name: Keratin, type I cytoskeletal 18
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430). Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.
    Isotype
    IgG
  • Target
    Alternative Name
    KRT18 (KRT18 Antibody Abstract)
    Background
    Keratin 18, mapped to 12q13.13, is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

    Synonyms: CK18 | CK-18 | CK 18 | CYK 18 | CYK18 | Cytokeratin 18 | Cytokeratin endo B | Cytokeratin-18 | K 18 | K18 | KA18 | Keratin 18 | Keratin D | Keratin-18 | Krt18 | P05783
    Gene ID
    3875
    UniProt
    P05783
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Li, Li, Sun, Wu, Wang: "Comparison of the effect of recombinant bovine wild and mutant lipopolysaccharide-binding protein in lipopolysaccharide-challenged bovine mammary epithelial cells." in: Cell stress & chaperones, Vol. 21, Issue 3, pp. 439-52, 2017 (PubMed).

    Zhang, Liu, Liu, Zhou, Song, Cao, An: "miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression." in: PLoS ONE, Vol. 12, Issue 7, pp. e0179783, 2017 (PubMed).

    Wu, Guo, Hui, Ye, Xiang, Wan, Zou, Ma, Sun, Yang, Guo: "Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage." in: Journal of veterinary science, Vol. 15, Issue 4, pp. 475-83, 2015 (PubMed).

    Xu, Jin, Min, Li, Deng, Wu, Lin, Chen, Zhang, Li, Wang, Zhu, Wang, Chu, Shen, Li, Mao: "Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival." in: Oncotarget, Vol. 6, Issue 4, pp. 2434-50, 2015 (PubMed).

    Yao, Guo, Wang, Xu, Zhang, Li, Lai: "The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency." in: Stem cells international, Vol. 2016, pp. 4148923, 2015 (PubMed).

    Hu, Zhou, Shi, Zhu, Wang, Zhang, Hu, Qian, Xu: "Mesenchymal stem-like cells isolated from human esophageal carcinoma and adjacent non-cancerous tissues." in: Oncology letters, Vol. 5, Issue 1, pp. 179-184, 2012 (PubMed).

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