KRT18 antibody (Keratin 18) (AA 204-430)

Details for Product anti-KRT18 Antibody No. ABIN5518769
Antigen
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Epitope
AA 204-430
34
33
26
17
16
15
11
9
8
8
7
7
5
5
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
882
224
214
126
95
64
64
52
48
37
35
33
33
33
24
14
11
9
6
3
2
2
2
1
1
Host
Rabbit
593
326
14
7
5
2
1
Clonality
Polyclonal
Conjugate
This KRT18 antibody is un-conjugated
55
41
18
11
11
11
11
11
11
10
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
8
3
3
3
3
3
2
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
562
399
367
365
240
193
170
86
77
76
41
40
30
17
14
11
2
1
1
1
Options
Purpose Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 18(KRT18) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Immunogen E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430). Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 18(KRT18) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: keratin 18
Protein Name: Keratin, type I cytoskeletal 18
Purification Immunogen affinity purified.
Plasmids, Primers & others Plasmids, Primers & others KRT18 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name KRT18 (KRT18 Antibody Abstract)
Background Keratin 18, mapped to 12q13.13, is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Synonyms: CK18 | CK-18 | CK 18 | CYK 18 | CYK18 | Cytokeratin 18 | Cytokeratin endo B | Cytokeratin-18 | K 18 | K18 | KA18 | Keratin 18 | Keratin D | Keratin-18 | Krt18 | P05783
Gene ID 3875
UniProt P05783
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Supplier Images
Image no. 1 for anti-Keratin 18 (KRT18) (AA 204-430) antibody (ABIN5518769) Cytokeratin 18 was detected in paraffin-embedded sections of rat intestine tissues us...
Image no. 2 for anti-Keratin 18 (KRT18) (AA 204-430) antibody (ABIN5518769) Cytokeratin 18 was detected in paraffin-embedded sections of rat kidney tissues using...
Image no. 3 for anti-Keratin 18 (KRT18) (AA 204-430) antibody (ABIN5518769) Western blot analysis of Cytokeratin 18 expression in rat liver extract ( Lane 1), mo...
Image no. 4 for anti-Keratin 18 (KRT18) (AA 204-430) antibody (ABIN5518769) Cytokeratin 18 was detected in paraffin-embedded sections of human intetsinal cancer ...
Image no. 5 for anti-Keratin 18 (KRT18) (AA 204-430) antibody (ABIN5518769) Cytokeratin 18 was detected in paraffin-embedded sections of human placenta tissues u...
Image no. 6 for anti-Keratin 18 (KRT18) (AA 204-430) antibody (ABIN5518769) Cytokeratin 18 was detected in paraffin-embedded sections of mouse intestine tissues ...
Image no. 7 for anti-Keratin 18 (KRT18) (AA 204-430) antibody (ABIN5518769) Cytokeratin 18 was detected in paraffin-embedded sections of mouse kidney tissues usi...
Product cited in: Li, Li, Sun, Wu, Wang: "Comparison of the effect of recombinant bovine wild and mutant lipopolysaccharide-binding protein in lipopolysaccharide-challenged bovine mammary epithelial cells." in: Cell stress & chaperones, Vol. 21, Issue 3, pp. 439-52, 2017 (PubMed).

Zhang, Liu, Liu, Zhou, Song, Cao, An: "miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression." in: PLoS ONE, Vol. 12, Issue 7, pp. e0179783, 2017 (PubMed).

Wu, Guo, Hui, Ye, Xiang, Wan, Zou, Ma, Sun, Yang, Guo: "Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage." in: Journal of veterinary science, Vol. 15, Issue 4, pp. 475-83, 2015 (PubMed).

Xu, Jin, Min, Li, Deng, Wu, Lin, Chen, Zhang, Li, Wang, Zhu, Wang, Chu, Shen, Li, Mao: "Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival." in: Oncotarget, Vol. 6, Issue 4, pp. 2434-50, 2015 (PubMed).

Yao, Guo, Wang, Xu, Zhang, Li, Lai: "The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency." in: Stem cells international, Vol. 2016, pp. 4148923, 2015 (PubMed).

Hu, Zhou, Shi, Zhu, Wang, Zhang, Hu, Qian, Xu: "Mesenchymal stem-like cells isolated from human esophageal carcinoma and adjacent non-cancerous tissues." in: Oncology letters, Vol. 5, Issue 1, pp. 179-184, 2012 (PubMed).