CPI-17 antibody (AA 30-126)

Catalog No. ABIN5518865

Product Details anti-CPI-17 Antibody

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Binding Specificity: AA 30-126

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPI-17 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Protein phosphatase 1 regulatory subunit 14A(PPP1R14A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein phosphatase 1 regulatory subunit 14A(PPP1R14A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein phosphatase 1 regulatory inhibitor subunit 14A
Protein Name: Protein phosphatase 1 regulatory subunit 14A

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126). Human CPI17 alpha shares 83.5% and 84.5% amino acid (aa) sequence identity with mouse and rat CPI17 alpha, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CPI-17

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Alternative Name: PPP1R14A (PPP1R14A Products)

Background: Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Synonyms: 17-KDa protein | CPI 17 alpha | CPI 17 | CPI 17alpha | CPI-17 | CPI17 | PPP1INL | Ppp1r14a | Q96A00

Gene ID: 94274

UniProt: Q96A00