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Aurora Kinase B antibody (AA 283-313)

Name: Aurora Kinase B antibody (AA 283-313)
SKU: ABIN5530328
Price: 644.88 USD
Availability: InStock

AURKB Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Antibody is used in Western blot to detect STK12 in mouse spleen tissue lysate. (Aurora Kinase B antibody (AA 283-313))

Western blot analysis of AURKB using rabbit polyclonal Aurora-B (ARK/STK12) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKB gene (Lane 2). (Aurora Kinase B antibody (AA 283-313))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (Aurora Kinase B antibody (AA 283-313))

IHC

3 images

Catalog No. ABIN5530328

Datasheet as PDF

Target See all Aurora Kinase B (AURKB) Antibodies

Aurora Kinase B (AURKB)
Binding Specificity
20

19

13

11

10

8

7

7

6

5

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 283-313
Reactivity
116

64

33

14

4

4

2

1

1

1

1

1
Human, Mouse
Host
112

23

3

1
Rabbit
Compatible Secondaries
Clonality
100

40
Polyclonal
Conjugate
79

10

7

6

4

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Aurora Kinase B antibody is un-conjugated
Application
83

59

43

34

15

14

14

13

8

6

4

3

3

3

2

2

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen

This Aurora-B (ARK/STK12) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the Central region of human Aurora-B (ARK/STK12).

Isotype

Ig Fraction

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anti-Aurora Kinase B (AURKB) (AA 1-90) antibody
AURKB Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 6H7 unconjugated

anti-Aurora Kinase B (AURKB) (Internal Region) antibody
AURKB Reactivity: Human, Monkey WB, ELISA, IHC, FM Host: Rabbit Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (AA 6-35) antibody
AURKB Reactivity: Human, Mouse, Rat, Primate WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) antibody
AURKB Reactivity: Human WB, ELISA, IHC, IP Host: Rabbit Monoclonal 4E5 unconjugated Recombinant Antibody

anti-Aurora Kinase B (AURKB) (pThr232) antibody
AURKB Reactivity: Human, Mouse, Rat, Monkey WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
AURKB Reactivity: Human IHC, StM Host: Mouse Monoclonal rAURKB-1592 unconjugated Recombinant Antibody

anti-Aurora Kinase B (AURKB) (AA 1-90) antibody
AURKB Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 6G8 unconjugated

anti-Aurora Kinase B (AURKB) (AA 1-255) antibody
AURKB Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
AURKB Reactivity: Human IHC, StM Host: Mouse Monoclonal AURKB-1592 unconjugated

anti-Aurora Kinase B (AURKB) (pThr232) antibody
AURKB Reactivity: Human, Mouse, Rat, Cow, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions

For Research Use only
Format

Liquid

Concentration

2 mg/mL

Buffer

Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target

Aurora Kinase B (AURKB)

Alternative Name

Aurora-B (AURKB Products)

Background

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. STK12 (Aurora kinase B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes

Molecular Weight

39 kDa

Gene ID

9212

UniProt

Q96GD4

Pathways

TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades