GATA4 antibody

Catalog No. ABIN5596711

Supplier Product No.: 200-301-p15

Product Details anti-GATA4 Antibody

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GATA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-301-p15

Supplier: Rockland

Purpose: GATA4 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93 % homology.

Characteristics: Synonyms: mouse anti-GATA4 Antibody, Transcription factor GATA-4, GATA-binding factor 4, GATA-4 Antibody

Purification: Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.

Immunogen Type: Recombinant Protein

Clone: 10D8-B10-D4

Isotype: IgG1 kappa

Application Details

Application Notes:

Application Note: Anti-GATA4 antibody has been tested by ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500-1:1000

ELISA Dilution: 1:2000-1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Alternative Name: GATA4 (GATA4 Products)

Background: Background: GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Gene ID: 2626

NCBI Accession: NP_002043

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis