BLM antibody (pSer1290)

Catalog No. ABIN5596799

Supplier Product No.: 600-401-j55

Product Details anti-BLM Antibody

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Binding Specificity: pSer1290

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-j55

Supplier: Rockland

Purpose: BLM phospho S1290 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human and mouse based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-BLM pS1290 Antibody, DNA helicase, RecQ-like type 2, RECQ2, RECQL3, Bloom syndrome protein

Purification: Anti-BLM pS1290 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human S1290 residue.

Immunogen:

Immunogen: Anti-BLM pS1290 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Bloom syndrome protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-BLM pS1290 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~159 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for BLM

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Alternative Name: BLM (BLM Products)

Background: Background: BLM pS1290 product is a member of the RecQ helicase protein family. The protein contains 1417 amino acids, including one ATP binding site, one DEAH box, and two putative nuclear localization signals, as well as phosphorylation sites. BLS protein is known to associate and be phosphorylated by the ATR (ataxia telangiectasia, rad3+) protein, in response to genetic stress. The Bloom's syndrome gene product is thought to play a role in the etiology of two major human health problems, each of which is a very common complication of Bloom's syndrome, namely cancer and diabetes. Anti-BLM pS1290 antibody is ideal for researchers interested in Epigenetics and Nuclear Signaling or DNA Damage & Repair research.

UniProt: P54132

Pathways: DNA Damage Repair