Collagen, Type I (COL1) antibody (Biotin)

Details for Product No. ABIN5596821
Antigen
  • collagen type III alpha 1 chain
  • COL3A1
Reactivity
Cow (Bovine), Human, Mammalian, Mouse (Murine), Rat (Rattus)
193
83
83
26
13
11
9
8
8
7
7
6
5
5
4
4
4
2
2
2
1
1
Host
Rabbit
180
64
37
Clonality
Polyclonal
Conjugate
Biotin
27
10
7
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
187
119
92
86
76
76
67
34
25
21
18
15
13
8
7
5
5
4
2
1
1
Options
Immunogen

Immunogen: Collagen Type I from human and bovine placenta.

Immunogen Type: Native Protein

Isotype IgG
Cross-Reactivity (Details) Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Purification This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Labeling Ratio 10-20
Antigen
Alternative Name COLLAGEN Type I
Background

Synonyms: Collagen Of Skin Tendon And Bone antibody, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, OI4 antibody, Osteogenesis Imperfecta Type IV antibody

Background: COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

Gene Name: COL1A1

Gene ID 1277
UniProt P02452
Application Notes

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-COLLAGEN Type I Antibody Biotin Conjugated is suitable for western blot, immunoprecipitatioin, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:3,000 - 1:6,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:3,000 - 1:6,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Collagen, Type I (COL1) antibody (Biotin) (ABIN5596821) Flow Cytometry of Anti-Collagen Type I Biotin Conjugated Antibody. Cells: mouse lung....
Image no. 2 for anti-Collagen, Type I (COL1) antibody (Biotin) (ABIN5596821) Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin at p...
Image no. 3 for anti-Collagen, Type I (COL1) antibody (Biotin) (ABIN5596821) Flow Cytometry of Rabbit Anti-Collagen 1 Antibody. Cells: primary adult human dermal ...
Image no. 4 for anti-Collagen, Type I (COL1) antibody (Biotin) (ABIN5596821) Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin at p...
Image no. 5 for anti-Collagen, Type I (COL1) antibody (Biotin) (ABIN5596821) Western Blot of Rabbit anti-Collagen I antibody. Lane 1: Wistar rat hepatic stellate ...
Product cited in: Heukels, van Hulst, van Nimwegen, Boorsma, Melgert, van den Toorn, Boomars, Wijsenbeek, Hoogsteden, von der Thüsen, Hendriks, Kool, van den Blink: "Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis." in: Respiratory research, Vol. 19, Issue 1, pp. 90, 2018 (PubMed).

Sinha, Hoshino, Hong, Kirkbride, Grega-Larson, Seiki, Tyska, Weaver: "Cortactin promotes exosome secretion by controlling branched actin dynamics." in: The Journal of cell biology, Vol. 214, Issue 2, pp. 197-213, 2017 (PubMed).

Collins, Chan-Li, Oh, Vigeland, Limjunyawong, Mitzner, Powell, Horton: "Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis." in: JCI insight, Vol. 1, Issue 4, pp. e83116, 2016 (PubMed).

Zhang, Maric, DiPrima, Khan, Orentas, Kaplan, Mackall: "Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer." in: Blood, Vol. 122, Issue 7, pp. 1105-13, 2013 (PubMed).

Did you look for something else?