COL1A1 antibody (Biotin)

Catalog No. ABIN5596821

Supplier Product No.: 600-406-103

Product Details anti-COL1A1 Antibody (Biotin)

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL1A1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Dot Blot (DB), Multiplex Assay (MA)

Supplier Product No.: 600-406-103

Supplier: Rockland

Purpose: Collagen Type I Antibody Biotin Conjugated

Cross-Reactivity (Details): Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Characteristics: Synonyms: rabbit anti-collagen type I antibody biotin conjugation, biotin conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1

Purification: This product has been prepared by immunoaffinity chromatography using immobilized antigens.

Immunogen:

Immunogen: Collagen Type I from human and bovine placenta.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-COLLAGEN Type I Antibody Biotin Conjugated has been tested by dot blot and Flow Cytometry and is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:3,000 - 1:6,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:3,000 - 1:6,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-COL1A1 antibody (Biotin) (ABIN5596821)

Heukels, van Hulst, van Nimwegen, Boorsma, Melgert, van den Toorn, Boomars, Wijsenbeek, Hoogsteden, von der Thüsen, Hendriks, Kool, van den Blink: "Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis." in: Respiratory research, Vol. 19, Issue 1, pp. 90, (2018) (PubMed).

Sinha, Hoshino, Hong, Kirkbride, Grega-Larson, Seiki, Tyska, Weaver: "Cortactin promotes exosome secretion by controlling branched actin dynamics." in: The Journal of cell biology, Vol. 214, Issue 2, pp. 197-213, (2017) (PubMed).

Collins, Chan-Li, Oh, Vigeland, Limjunyawong, Mitzner, Powell, Horton: "Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis." in: JCI insight, Vol. 1, Issue 4, pp. e83116, (2016) (PubMed).

Zhang, Maric, DiPrima, Khan, Orentas, Kaplan, Mackall: "Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer." in: Blood, Vol. 122, Issue 7, pp. 1105-13, (2013) (PubMed).

Target Details for COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Alternative Name: COL1A1 (COL1A1 Products)

Background: Background: COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding