COL1A1 antibody (FITC)

Catalog No. ABIN5596822

Supplier Product No.: 600-402-103

Product Details anti-COL1A1 Antibody (FITC)

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivity: Human, Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL1A1 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Supplier Product No.: 600-402-103

Supplier: Rockland

Purpose: Collagen Type I Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Characteristics: Synonyms: rabbit anti-collagen type I antibody fluorescein conjugation, FITC conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1

Purification: This product has been prepared by immunoaffinity chromatography using immobilized antigens.

Immunogen:

Immunogen: Collagen Type I from human and bovine placenta.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 2-7

Application Details

Application Notes:

Flow Cytometry Dilution: 5 μg/mL

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-COLLAGEN Type I Fluorescein Conjugated Antibody is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:3,000 - 1:6,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:3,000 - 1:6,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 50μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-COL1A1 antibody (FITC) (ABIN5596822)

Liu, Pan, Cao, Shu, Sun, Lu, Liang, Zhang, Zhu, Li, Zhang: "IL-32γ promotes integrin αvβ6 expression through the activation of NF-κB in HSCs." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3880-3886, (2017) (PubMed).

Target Details for COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Alternative Name: COL1A1 (COL1A1 Products)

Background: Background: COLLAGEN Type I Fluorescein Conjugated Antibody is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding