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Collagen, Type I (COL1) antibody (HRP) Primary Antibody

COL1 Reactivity: Cow, Human, Mammalian, Mouse, Rat ELISA, IHC, IP, WB Host: Rabbit Polyclonal
Catalog No. ABIN5596823
$625.50
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50 μg
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  • Target
    Reactivity
    Cow, Human, Mammalian, Mouse, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    HRP
    Application
    ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
    Cross-Reactivity (Details)
    Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    Purification
    Anti-Collagen I has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
    Immunogen

    Immunogen: Collagen Type I from human and bovine placenta.

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:50 - 1:200

    Application Note: Anti-COLLAGEN Type I Peroxidase Conjugated Antibody is suitable for western blot, immunoprecipitatioin, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:3,000 - 1:6,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:3,000 - 1:6,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL to 20 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
    Expiry Date
    12 months
  • Target
    Alternative Name
    COLLAGEN Type I
    Background

    Synonyms: Collagen Of Skin Tendon And Bone antibody, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, OI4 antibody, Osteogenesis Imperfecta Type IV antibody

    Background: COLLAGEN Type I Peroxidase Conjugated Antibody is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

    Gene Name: COL1A1

    Gene ID
    1277
    UniProt
    P02452
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