COL2A1 antibody (FITC)

Catalog No. ABIN5596827

Supplier Product No.: 600-402-104

Product Details anti-COL2A1 Antibody (FITC)

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2A1 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM), Flow Cytometry (FACS)

Supplier Product No.: 600-402-104

Supplier: Rockland

Purpose: Collagen Type II Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Characteristics: Synonyms: rabbit anti-Collagen Type II antibody fluorescein conjugation, FITC conjugated rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody

Purification: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.

Immunogen:

Immunogen: Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Collagen Type II Fluorescein Conjugated Antibody is suitable for western blotting, IP, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: User Optimized

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 50μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Alternative Name: COL2A1 (COL2A1 Products)

Background: Background: Anti-Collagen Type II Fluorescein Conjugated Antibody detects Type-II collagen which is the basis for articular cartilage and hyaline cartilage. It makes up 50 % of all protein in cartilage and 85-90 % of collagen of articular cartilage. Type II collagen does form fibrils which allow cartilage to entrap the proteoglycan aggregate as well as provide tensile strength to the tissue. Anti-Collagen Type II antibody is ideal for investigators involved in Immunology, Neuroscience and Signal Transduction research.

Gene ID: 1280

NCBI Accession: NP_001835

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding