MYL12A antibody (N-Term)

Catalog No. ABIN5596872

Supplier Product No.: 100-401-416

Product Details anti-MYL12A Antibody

Target: MYL12A (Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL12A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 100-401-416

Supplier: Rockland

Purpose: Myosin phospho S19/phospho S20 Antibody

Cross-Reactivity (Details): This antiserum is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein.

Characteristics: Synonyms: rabbit anti-Myosin pS19/pS20 Antibody, Myosin regulatory light chain 12A, Myosin regulatory light chain MRLC3, Myosin regulatory light chain 2, nonsarcomeric, Myosin RLC, MLC-2B

Purification: Anti-Myosin pS19/pS20 antibody is directed against the regulatory light chain of smooth and non-muscle myosin.

Sterility: Sterile filtered

Immunogen:

Immunogen: Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-Myosin pS19/pS20 phospho specific polyclonal antibody was tested by ELISA and immunoblotting.   Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes.  The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.  Although not tested, this antibody is likely functional by immunohistochemistry and immunoprecipitation.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 70 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MYL12A

Target: MYL12A (Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A))

Alternative Name: MYL12A (MYL12A Products)

Background: Background: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  This antibody is specific for the phosphorylated form of myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.  This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site.  Human and mouse have almost identical sequences.  In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

Gene ID: 10627

UniProt: P19105