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CHRNA3 antibody (AA 32-240)

Name: CHRNA3 antibody (AA 32-240)
Brand: antibodies-online
SKU: ABIN5611357
Price: 533.5 USD
Availability: InStock

CHRNA3 Reactivity: Human ELISA, ICC, FACS Host: Mouse Monoclonal 6D3A10 unconjugated

Western blot analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (CHRNA3 antibody (AA 32-240))

Flow cytometric analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red). (CHRNA3 antibody (AA 32-240))

FACS

Immunofluorescence analysis of Hela cells using CHRNA3 mouse mAb (green). (CHRNA3 antibody (AA 32-240))

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (CHRNA3 antibody (AA 32-240))

ELISA

Immunofluorescence analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). (CHRNA3 antibody (AA 32-240))

Western blot analysis using CHRNA3 mAb against HEK293 (1) and CHRNA3 (AA: 32-240)-hIgGFc transfected HEK293 (2) cell lysate. (CHRNA3 antibody (AA 32-240))

Flow cytometric analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red). (CHRNA3 antibody (AA 32-240))

FACS

7 images

(1 reference)

Catalog No. ABIN5611357

Datasheet as PDF

Target See all CHRNA3 Antibodies

CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))
Binding Specificity
15

8

6

3

2

2

1

1

1

1

1
AA 32-240
Reactivity
55

20

19

7

6

4

3

3

2

1

1

1
Human
Host
54

1
Mouse
Compatible Secondaries
Clonality
54

1
Monoclonal
Conjugate
28

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CHRNA3 antibody is un-conjugated
Application
46

17

16

13

13

7

4

4

4

2

1

1
ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose

CHRNA3 Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human CHRNA3 (AA: 32-240) expressed in E. Coli.

Clone

6D3A10

Isotype

IgG1
anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (AA 32-240) antibody
CHRNA3 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (AA 25-52), (N-Term) antibody
CHRNA3 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB25608 unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (AA 26-75) antibody
CHRNA3 Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (AA 35-84) antibody
CHRNA3 Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Monkey, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (Center) antibody
CHRNA3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (N-Term) antibody
CHRNA3 Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig WB Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) antibody
CHRNA3 Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (N-Term) antibody
CHRNA3 Reactivity: Human, Dog WB Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (N-Term) antibody
CHRNA3 Reactivity: Human, Mouse, Rat, Cow, Dog WB Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) antibody
CHRNA3 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Kulbatskii, Shenkarev, Bychkov, Loktyushov, Shulepko, Koshelev, Povarov, Popov, Peigneur, Chugunov, Kozlov, Sharonova, Efremov, Skrebitsky, Tytgat, Kirpichnikov, Lyukmanova: "Human Three-Finger Protein Lypd6 Is a Negative Modulator of the Cholinergic System in the Brain." in: Frontiers in cell and developmental biology, Vol. 9, pp. 662227, (2021) (PubMed).
Target

CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Alternative Name

CHRNA3 (CHRNA3 Products)

Background

Description: This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.
Aliases: LNCR2, PAOD2, NACHRA3

Molecular Weight

57.5kDa

Gene ID

1136

HGNC

1136

UniProt

P32297

Pathways

Synaptic Membrane