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Fibronectin antibody

Reactivity: Cow IF, ELISA, IHC (p), RIA Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN5680161

Datasheet as PDF

Target See all Fibronectin Antibodies

Fibronectin
Reactivity
108

54

53

24

15

9

4

2

2

2

1
Cow
Host
72

62

5

2
Rabbit
Compatible Secondaries
Clonality
70

69

1
Polyclonal
Conjugate
63

18

10

7

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Fibronectin antibody is un-conjugated
Application
75

43

42

28

26

19

18

18

18

17

13

12

10

7

7

5

4

2

2

1

1

1

1

1

1

1

1

1
Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Specificity

Bovine fibronectin 100 % , bovine collagen type I and III <0.1 % (RIA at 1:10000 dilution)

Cross-Reactivity (Details)

Cattle, cross-reaction with dog

Characteristics

This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Bovine fibronectin 100 % , bovine collagen type I and III <0.1 % (RIA at 1:10000 dilution).

Purification

Affinity purified

Immunogen

Purified fibronectin from bovine plasma

Isotype

IgG

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Reactivity: Chicken WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Fibronectin (AA 355-558) antibody
Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C2 unconjugated

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Reactivity: Human, Mouse, Rat WB, IHC (p), IF (p) Host: Mouse Monoclonal 1B9 unconjugated

anti-Fibronectin (AA 2206-2337) antibody
Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Fibronectin antibody
Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine skin or liver,IFA, IHC(P), ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:80, IHC(P) 1:500 (Envision), ELISA 1:200 (OD>500)

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.

Concentration

1 mg/mL

Buffer

Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!

Preservative

Without preservative

Storage

-20 °C
Target

Fibronectin

Abstract

Fibronectin Products