SPOP-B antibody

Catalog No. ABIN5701294

Product Details anti-SPOP-B Antibody

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPOP-B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Immunogen: speckle-type POZ protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for SPOP-B

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Alternative Name: SPOP (SPOP-B Products)

Background: Synonyms:HIB homolog 1, Roadkill homolog 1, speckle type POZ protein, SPOP, TEF2 Background:Component of a cullin-RING-based BCR(BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1, this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR(BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.

Gene ID: 8405

UniProt: O43791