SPOP-B antibody (C-Term)

Catalog No. ABIN2784150

Product Details anti-SPOP-B Antibody

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPOP-B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YHASDVLETS GWKSMVVSHP HLVAEAYRSL ASAQCPFLGP PRKRLKQS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SPOP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SPOP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 374 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPOP-B

Target: SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Alternative Name: SPOP (SPOP-B Products)

Synonyms: BTBD32 antibody, TEF2 antibody, AI315626 antibody, Pcif1 antibody, zgc:64140 antibody, spop antibody, tef2 antibody, speckle type BTB/POZ protein antibody, speckle-type POZ protein antibody, speckle type POZ protein S homeolog antibody, SPOP antibody, Spop antibody, spop antibody, spop.S antibody

Background: SPOP may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
Alias Symbols: TEF2
Protein Interaction Partner: CUL3, SPOP, DEK, GLI2, AR, ATM, RXRB, MYD88, DAXX, NCOA3, SRRM1, GIT2, FADD, PIAS1, UBC, UBE2E3, PIP4K2B, ESR1, BMI1, RTN3, H2AFY, DPPA2, ZBTB16, HCST, TRAF1, PDX1, DDIT3, TRAF6, SIRT2, DOK2, PIP5K1B, GDI1, KPNA6,
Protein Size: 374

Molecular Weight: 42 kDa

Gene ID: 8405

NCBI Accession: NM_001007226, NP_001007227

UniProt: O43791