EIF3B antibody (AA 100-350)

Catalog No. ABIN6140053

Product Details anti-EIF3B Antibody

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Binding Specificity: AA 100-350

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Sequence: PVPAQGEAPG EQARDERSDS RAQAVSEDAG GNEGRAAEAE PRALENGDAD EPSFSDPEDF VDDVSEEELL GDVLKDRPQE ADGIDSVIVV DNVPQVGPDR LEKLKNVIHK IFSKFGKITN DFYPEEDGKT KGYIFLEYAS PAHAVDAVKN ADGYKLDKQH TFRVNLFTDF DKYMTISDEW DIPEKQPFKD LGNLRYWLEE AECRDQYSVI FESGDRTSIF WNDVKDPVSI EERARWTETY VRWSPKGTYL A

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human eIF3B (NP_003742.2).

Isotype: IgG

Application Details

Application Notes: WB,1:1000 - 1:2000,IF,1:50 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for EIF3B

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Alternative Name: EIF3B (EIF3B Products)

Background: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3 complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.,EIF3B,EIF3-ETA,EIF3-P110,EIF3-P116,EIF3S9,PRT1,Epigenetics & Nuclear Signaling,EIF3B

Molecular Weight: 92 kDa/99 kDa

Gene ID: 8662

UniProt: P55884

Pathways: Ribonucleoprotein Complex Subunit Organization