EpCAM antibody (Extracellular Domain) (CF®594)

Catalog No. ABIN6158988

Product Details anti-EpCAM Antibody (CF®594)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Binding Specificity: AA 77-202, Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is conjugated to CF®594

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat)

Purpose: Mouse Monoclonal anti-Ep-CAM / CD326 (EGP40/1798), CF594 Conjugate

Characteristics: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human EpCAM protein fragment from the extracellular domain (aa77-202) (exact sequence is proprietary)

Clone: EGP40-1798

Isotype: IgG2b kappa

Application Details

Application Notes: ELISA 2-4 μg/mL for coating (order Ab without BSA)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HT29 cells or Ovarian carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: Ep-CAM / CD326 (EPCAM Products)

Molecular Weight: 40-43 kDa

Gene ID: 4072, 542050

UniProt: P16422