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ANO1 antibody (Anoctamin 1, Calcium Activated Chloride Channel) (CF®568) Primary Antibody

ANO1 Reactivity: Human FACS, IF, IHC (f) Host: Mouse Monoclonal DG1-447 CF®568
Catalog No. ABIN6172944
$270.77
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  • Target See all ANO1 Antibodies
    ANO1
    Reactivity
    • 156
    • 27
    • 22
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Human
    Host
    • 86
    • 73
    Mouse
    Clonality
    • 95
    • 64
    Monoclonal
    Conjugate
    • 65
    • 12
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ANO1 antibody is conjugated to CF®568
    Application
    • 77
    • 72
    • 58
    • 54
    • 36
    • 29
    • 18
    • 13
    • 13
    • 9
    • 8
    • 5
    • 4
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-DOG-1 (DG1/447), CF568 Conjugate
    Characteristics
    Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4 % vs. 94.7 %. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human DOG-1 protein
    Clone
    DG1-447
    Isotype
    IgG1 kappa
  • Application Notes
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells
    • Optimal dilution for a specific application should be determined by user
    Comment

    Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    ANO1
    Alternative Name
    DOG-1 (ANO1 Products)
    Synonyms
    DOG1, ORAOV2, TAOS2, TMEM16A, Tmem16a, anoctamin 1, anoctamin 1, calcium activated chloride channel, Ano1, ANO1
    Background
    Anoctamin 1, Calcium Activated Chloride Channel, Discovered On Gastrointestinal Stromal Tumors Protein 1,TAOS2, ORAOV2, TMEM16A
    Molecular Weight
    ~114 kDa
    Gene ID
    55107, 503074
    UniProt
    Q5XXA6
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