Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

KRT18 antibody (Keratin 18) Primary Antibody

KRT18 Reactivity: Human FACS, IF, IHC (f) Host: Mouse Monoclonal KRT18-834
Catalog No. ABIN6179389
$492.31
Plus shipping costs $45.00
50 μL
local_shipping Shipping to: United States
Delivery in 3 to 4 Business Days
  • Target
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This KRT18 antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-Cytokeratin 18 (KRT18/834), Purified, BSA-free
    Characteristics
    This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Purification
    Purified
    Immunogen
    Recombinant full-length human KRT18 protein
    Clone
    KRT18-834
    Isotype
    IgG1
  • Target
    Alternative Name
    Cytokeratin 18 (KRT18 Antibody Abstract)
    Background
    Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18
    Molecular Weight
    45 kDa
    Gene ID
    3875, 406013
    UniProt
    P05783
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
  • Application Notes
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Does not react with mouse, rat, sheep, hamster, cow, dog or pig, others not known
    • Optimal dilution for a specific application should be determined by user
    Comment

    MCF-7, HeLa, A431 cells or Breast Cancer

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS (no BSA, no azide)
    Preservative
    Azide free
You are here:
help Support