SLUG antibody (AA 98-129)

Catalog No. ABIN6242822

Product Details anti-SLUG Antibody

Target: SLUG

Binding Specificity: AA 98-129

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLUG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Predicted Reactivity: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SLUG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-129 amino acids from the Central region of human SLUG.

Clone: RB01398

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:2000. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-SLUG antibody (ABIN6242822)

Carpenter, Paw, Dewhirst, Lo: "Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells." in: Oncogene, Vol. 34, Issue 5, pp. 546-57, (2015) (PubMed).

Ferrari-Amorotti, Fragliasso, Esteki, Prudente, Soliera, Cattelani, Manzotti, Grisendi, Dominici, Pieraccioli, Raschellà, Chiodoni, Colombo, Calabretta: "Inhibiting interactions of lysine demethylase LSD1 with snail/slug blocks cancer cell invasion." in: Cancer research, Vol. 73, Issue 1, pp. 235-45, (2013) (PubMed).

Lemma, Karihtala, Haapasaari, Jantunen, Soini, Bloigu, Pasanen, Turpeenniemi-Hujanen, Kuittinen: "Biological roles and prognostic values of the epithelial-mesenchymal transition-mediating transcription factors Twist, ZEB1 and Slug in diffuse large B-cell lymphoma." in: Histopathology, Vol. 62, Issue 2, pp. 326-33, (2012) (PubMed).

Target Details for SLUG

Target: SLUG

Abstract: SLUG Products

Synonyms: Slug antibody, Slugh antibody, Snail2 antibody, SLUG antibody, SLUGH antibody, SLUGH1 antibody, SNAIL2 antibody, WS2D antibody, snail family zinc finger 2 antibody, snail family transcriptional repressor 2 antibody, Snai2 antibody, SNAI2 antibody

Background: SLUG is a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporadic cases of neural tube defects.

Molecular Weight: 29986

NCBI Accession: NP_003059

UniProt: O43623