Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) antibody

Details for Product No. ABIN6257658
Antigen
  • CBP80
  • CG7035
  • Dmel\\CG7035
  • EG:84H4.3
  • ncbp
  • sto1
  • cbp80
  • AU014645
  • AW538051
  • NCBP
  • Sto1
  • ncbp1
  • ABA HYPERSENSITIVE 1
  • ATCBP80
  • CAP-BINDING PROTEIN 80
  • ENS
  • ENSALADA
  • NUCLEAR CAP-BINDING PROTEIN CBP80
  • T10F5.8
  • T10F5_8
  • cap binding protein 80
  • nuclear cap binding protein subunit 1
  • nuclear cap binding protein subunit 1 S homeolog
  • nuclear cap binding protein subunit 1, 80kDa
  • nuclear cap binding protein subunit 1 L homeolog
  • ARM repeat superfamily protein
  • Nuclear cap-binding protein subunit 1-A
  • Nuclear cap-binding protein subunit 1
  • Cbp80
  • ncbp1
  • ncbp1.S
  • NCBP1
  • Ncbp1
  • ncbp1.L
  • ABH1
  • nbp1a
  • ncbp-1
Alternatives
anti-Human Nuclear Cap Binding Protein Subunit 1, 80kDa antibody for ELISA
Reactivity
Human, Mouse (Murine), Rat (Rattus)
76
31
27
5
4
4
3
2
2
2
1
1
1
Host
Rabbit
69
7
Clonality
Polyclonal
Conjugate
Un-conjugated
4
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
60
39
18
8
7
6
4
1
Options
Immunogen A synthesized peptide
Isotype IgG
Specificity NCBP1 Antibody detects endogenous levels of total NCBP1
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Antigen
Alternative Name NCBP1 (NCBP1 Antibody Abstract)
Background

Description: Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858).

Gene: NCBP1

Molecular Weight 80 kDa
Gene ID 4686
UniProt Q09161
Pathways Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process
Application Notes WB 1:500~1:1000, IF/ICC 1:100-1:500
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) antibody (ABIN6257658) ABIN6274323 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeab...
Western Blotting (WB) image for anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) antibody (ABIN6257658) Western blot analysis of extracts from HT-29 cells using NCBP1 antibody.The lane on t...
Did you look for something else?