NCBP1 antibody (N-Term)

Catalog No. ABIN7184484

Product Details anti-NCBP1 Antibody

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human, Mouse, Rat

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from N-terminal of Human NCBP1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for NCBP1

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Alternative Name: NCBP1 (NCBP1 Products)

Synonyms: CBP80 antibody, CG7035 antibody, Dmel\\CG7035 antibody, EG:84H4.3 antibody, ncbp antibody, sto1 antibody, cbp80 antibody, AU014645 antibody, AW538051 antibody, NCBP antibody, Sto1 antibody, ncbp1 antibody, ABA HYPERSENSITIVE 1 antibody, ATCBP80 antibody, CAP-BINDING PROTEIN 80 antibody, ENS antibody, ENSALADA antibody, NUCLEAR CAP-BINDING PROTEIN CBP80 antibody, T10F5.8 antibody, T10F5_8 antibody, cap binding protein 80 antibody, nuclear cap binding protein subunit 1 antibody, nuclear cap binding protein subunit 1 S homeolog antibody, nuclear cap binding protein subunit 1, 80kDa antibody, nuclear cap binding protein subunit 1 L homeolog antibody, ARM repeat superfamily protein antibody, Nuclear cap-binding protein subunit 1-A antibody, Nuclear cap-binding protein subunit 1 antibody, Cbp80 antibody, ncbp1 antibody, ncbp1.S antibody, NCBP1 antibody, Ncbp1 antibody, ncbp1.L antibody, ABH1 antibody, nbp1a antibody, ncbp-1 antibody

Background:

Background: Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.

Izaurralde E., Cell 78:657-668(1994).
Kataoka N., Nucleic Acids Res. 22:3861-3865(1994).
Humphray S.J., Nature 429:369-374(2004).

Aliases: 80 kDa nuclear cap-binding protein antibody, CBP80 antibody, MGC2087 antibody, NCBP 80 kDa subunit antibody, NCBP antibody, ncbp1 antibody, NCBP1_HUMAN antibody, nuclear cap binding protein subunit 1 antibody, nuclear cap binding protein subunit 1, 80 kDa antibody, Nuclear cap-binding protein subunit 1 antibody, Sto1 antibody

UniProt: Q09161

Pathways: Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process