ANO1 antibody

Catalog No. ABIN6297579

Product Details anti-ANO1 Antibody

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Purpose: Mouse anti-Human TMEM16A Antibody (DOG1) [Sodium Azide Free]

Specificity: Cell Surface and Cytoplasmic

Purification: Recombinant human TMEM16A/DOG1 protein was used as the immunogen for this TMEM16A antibody.

Immunogen: Recombinant human TMEM16A/DOG1 protein was used as the immunogen for this TMEM16A antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: Anoctamin-1 (ANO1 Products)

Synonyms: DOG1 antibody, ORAOV2 antibody, TAOS2 antibody, TMEM16A antibody, Tmem16a antibody, anoctamin 1 antibody, anoctamin 1, calcium activated chloride channel antibody, Ano1 antibody, ANO1 antibody

Background:

Target Description: Expression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GIST 's), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for TMEM16A has been reported in 97.8 percent of scorable GIST 's, including all c-kit negative GIST 's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GIST 's is nearly identical: 94.4 % vs. 94.7 %.

Gene Symbol: ANO1///DOG1///ORAOV2///TAOS2///TMEM16A

Gene ID: 55107

UniProt: Q5XXA6