CD43 antibody (Sialophorin)

Details for Product anti-SPN Antibody No. ABIN6297682
Antigen
  • CD43
  • GALGP
  • GPL115
  • LSN
  • A630014B01Rik
  • Cd43
  • Galgp
  • Ly-48
  • Ly48
  • Lsn
  • Lsn1
  • gpL115
  • sialophorin
  • SPN
  • Spn
Reactivity
Human
528
44
27
2
1
Host
Mouse
410
160
14
Clonality
Monoclonal
Conjugate
This CD43 antibody is un-conjugated
52
47
41
22
16
15
10
6
6
6
6
6
6
6
6
6
6
6
6
6
3
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
397
255
214
205
118
107
82
54
36
22
19
5
3
2
1
Options
Purpose Mouse anti-Human CD43 Antibody [Sodium Azide Free]
Immunogen Stimulated human leukocytes were used as the immunogen for the CD43 antibody.
Specificity Cell surface
Purification Stimulated human leukocytes were used as the immunogen for the CD43 antibody.
Plasmids, Primers & others Plasmids, Primers & others CD43 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Leukosialin (SPN Antibody Abstract)
Background

Target Description: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Gene Symbol: SPN///CD43

Gene ID 6693
UniProt P16150
Pathways Regulation of Leukocyte Mediated Immunity
Application Notes Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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