Golgi antibody

Details for Product No. ABIN6297711
Antigen
Reactivity
Human
15
Host
Mouse
12
3
Clonality
Monoclonal
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
12
9
9
6
6
3
Options
Purpose Mouse anti-Human Golgi Antibody [Sodium Azide Free]
Immunogen SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.
Specificity Golgi complex in cytoplasm
Purification SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.
Antigen
Background

Target Description: This mAb recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.

Application Notes Flow Cytometry (0.5-1 μg/million cells in 0.1ml)
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL,ICC (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Restrictions For Research Use only
Buffer In 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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