Golgi antibody

Catalog No. ABIN6297711

Product Details

Target: Golgi

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purpose: Mouse anti-Human Golgi Antibody [Sodium Azide Free]

Specificity: Golgi complex in cytoplasm

Purification: SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.

Immunogen: SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.

Application Details

Application Notes: Flow Cytometry (0.5-1 μg/million cells in 0.1ml)
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL,ICC (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details

Target: Golgi

Background:

Target Description: This mAb recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.