Golgi antibody

Details for Product No. ABIN6297710
Antigen
Reactivity
Human
15
Host
Mouse
12
3
Clonality
Monoclonal
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
12
9
9
6
6
3
Options
Purpose Mouse anti-Human Golgi Antibody [Sodium Azide Free]
Immunogen SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.
Specificity Golgi complex in cytoplasm
Purification SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.
Antigen
Background

Target Description: This mAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

Application Notes Flow Cytometry (0.5-1 μg/million cells in 0.1ml)
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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