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Golgi antibody

Reactivity: Human FACS, IF, WB, ICC, IHC Host: Mouse Monoclonal unconjugated
Catalog No. ABIN6297710
  • Target
    Golgi
    Reactivity
    Human
    Host
    • 4
    • 2
    Mouse
    Clonality
    • 6
    Monoclonal
    Conjugate
    • 6
    Un-conjugated
    Application
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
    Purpose
    Mouse anti-Human Golgi Antibody [Sodium Azide Free]
    Specificity
    Golgi complex in cytoplasm
    Purification
    SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.
    Immunogen
    SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.
  • Application Notes
    Flow Cytometry (0.5-1 μg/million cells in 0.1ml)
    Immunofluorescence: 0.5-1 μg/mL
    Western blot: 0.5-1 μg/mL
    Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)
    Restrictions
    For Research Use only
  • Buffer
    In 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • Target
    Golgi
    Background

    Target Description: This mAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

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