CD74 antibody (HLA-DR-gamma)

Details for Product anti-CD74 Antibody No. ABIN6297715
Target
Reactivity
Human, Mouse
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22
Host
Mouse
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2
Clonality
Monoclonal
Conjugate
This CD74 antibody is un-conjugated
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12
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4
3
3
3
3
3
3
1
1
1
1
1
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1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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Options
Purpose Mouse anti-Human/Mouse CD74 Antibody [Sodium Azide Free]
Immunogen SU-DHL-4 lymphoma cells were used as the immunogen for this anti-CD74 antibody.
Specificity Cell surface and paranuclear globular
Purification SU-DHL-4 lymphoma cells were used as the immunogen for this anti-CD74 antibody.
Target
Alternative Name HLA class II histocompatibility antigen gamma chain (CD74 Antibody Abstract)
Background

Target Description: CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Gene Symbol: CD74///DHLAG

Gene ID 296
UniProt P04233
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
Application Notes Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.