Nucleolin antibody (NCL)

Details for Product anti-NCL Antibody No. ABIN6297718
Antigen
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Human
239
62
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Mouse
130
116
1
Clonality
Monoclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
220
141
132
98
72
60
43
25
21
17
13
9
9
2
2
1
1
Options
Purpose Mouse anti-Human Nucleolin Antibody Cocktail [IHC only]
Immunogen SUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.
Specificity Nucleoli
Purification SUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.
Plasmids, Primers & others Plasmids, Primers & others Nucleolin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background

Target Description: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Gene Symbol: NCL

Gene ID 4691
UniProt P19338
Pathways Ribonucleoprotein Complex Subunit Organization
Application Notes Prediluted format : incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, For IHC use only
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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