Nucleolin antibody
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- Target See all Nucleolin (NCL) Antibodies
- Nucleolin (NCL)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Nucleolin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Purpose
- Mouse anti-Human NCL Antibody [IHC only]
- Specificity
- Nucleoli
- Purification
- SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody.
- Immunogen
- SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody.
- Top Product
- Discover our top product NCL Primary Antibody
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- Application Notes
- Prediluted format : incubate for 30 min at RT (2)
- Restrictions
- For Research Use only
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- Buffer
- Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, For IHC use only
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Target
- Nucleolin (NCL)
- Alternative Name
- Nucleolin (NCL Products)
- Background
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Target Description: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Gene Symbol: NCL
- Gene ID
- 4691
- UniProt
- P19338
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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