CD20 antibody (Membrane-Spanning 4-Domains, Subfamily A, Member 1)

Details for Product anti-MS4A1 Antibody No. ABIN6297751
Target
Reactivity
Human
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Host
Mouse
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4
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Clonality
Monoclonal
Conjugate
This CD20 antibody is un-conjugated
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Application
Flow Cytometry (FACS), Functional Studies (Func), Immunofluorescence (IF)
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Options
Purpose Mouse anti-Human CD20 Antibody [Sodium Azide Free]
Immunogen The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.
Specificity Predominantly cell surface with some cytoplasmic
Purification The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.
Target
Alternative Name B-lymphocyte antigen CD20 (MS4A1 Antibody Abstract)
Background

Target Description: This antibody recognizes a protein of 33-37 kDa, identified as CD20 (Workshop V, Code CD20.12). The antibody recognizes the extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.

Gene Symbol: MS4A1///CD20

Gene ID 931
UniProt P11836
Application Notes Functional Testing: order azide-free format
FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.