CD20 antibody (Membrane-Spanning 4-Domains, Subfamily A, Member 1)

Details for Product anti-MS4A1 Antibody No. ABIN94025
Antigen
Reactivity
Human, Pig (Porcine), Cow (Bovine)
762
81
48
34
14
10
9
7
7
6
5
3
1
Host
Mouse
590
181
8
5
3
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD20 antibody is un-conjugated
62
56
44
42
14
10
9
7
7
6
6
6
6
6
6
6
6
6
6
6
6
5
5
5
5
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
501
184
181
174
109
100
93
49
43
23
20
19
14
13
10
7
6
3
2
2
2
1
1
Options
Immunogen Raji human Burkitt's lymphoma cell line
Clone MEM-97
Isotype IgG1
Specificity The antibody MEM-97 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
HLDA V, WS Code B CD20.9
Cross-Reactivity (Details) Human, Porcine, Bovine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD20 (MS4A1 Antibody Abstract)
Background CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (ABIN94025) Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt ly...
Image no. 2 for anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (ABIN94025) Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and...
Product cited in: Faldyna, Samankova, Leva, Cerny, Oujezdska, Rehakova, Sinkora: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, 2007 (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Szöllósi, Horejsí, Bene, Angelisová, Damjanovich: "Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2939-46, 1996 (PubMed).

Strobl, Takimoto, Majdic, Fritsch, Scheinecker, Höcker, Knapp: "Myeloperoxidase expression in CD34+ normal human hematopoietic cells." in: Blood, Vol. 82, Issue 7, pp. 2069-78, 1993 (PubMed).