CA9 antibody

Catalog No. ABIN6297760

Product Details anti-CA9 Antibody

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse anti-Human/Horse CAIX Antibody Carbonic Anhydrase IX [Sodium Azide Free]

Specificity: Cell surface and cytoplasmic

Purification: The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX / CAIX antibody.

Immunogen: The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX / CAIX antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: Carbonic anhydrase 9 (CA9 Products)

Synonyms: CA9 antibody, CAIX antibody, Ca9 antibody, MN/CA9 antibody, MN antibody, carbonic anhydrase 9 antibody, CA9 antibody, CAH9 antibody, Car9 antibody

Background:

Target Description: This antibody recognizes a glycoprotein of ~200 kDa, identified as renal cell carcinoma protein, carbonic anhydrase IX / CAIX, or gp200. Its epitope resides in the carbohydrate domain of the protein. The CAIX antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, it is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This CAIX antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation, especially those showing tubular differentiation.

Gene Symbol: CA9///G250///MN

Gene ID: 768

UniProt: Q16790