Carbonic Anhydrase IX (CA9) antibody

Details for Product No. ABIN6297761
Antigen
  • CA9
  • CAIX
  • Ca9
  • MN/CA9
  • MN
  • carbonic anhydrase 9
  • CA9
  • CAH9
  • Car9
Reactivity
Horse (Equine), Human
240
57
37
30
3
Host
Mouse
138
120
Clonality
Monoclonal
Conjugate
Un-conjugated
12
5
5
4
4
4
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
182
86
82
77
68
46
21
20
13
12
9
8
5
3
1
Options
Purpose Mouse anti-Human/Horse Carbonic Anhydrase IX Antibody [Sodium Azide Free]
Immunogen The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX antibody.
Specificity Cell surface and cytoplasmic
Purification The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX antibody.
Antigen
Alternative Name Carbonic anhydrase 9 (CA9 Antibody Abstract)
Background

Target Description: Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/CA9/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This mAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Gene Symbol: CA9///G250///MN

Gene ID 768
UniProt Q16790
Application Notes Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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