IGHM antibody (Immunoglobulin Heavy Constant mu)

Details for Product anti-IGHM Antibody No. ABIN6297768
Target
Reactivity
Human
25
1
1
1
Host
Mouse
17
9
Clonality
Monoclonal
Conjugate
This IGHM antibody is un-conjugated
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
18
14
13
10
10
8
4
2
2
1
Options
Purpose Mouse anti-Human IgM Antibody [Sodium Azide Free]
Immunogen The Mu heavy chain of human Ig was used as the immunogen for the Anti-IgM antibody.
Specificity Cytoplasm, cell surface and secreted
Purification The Mu heavy chain of human Ig was used as the immunogen for the Anti-IgM antibody.
Target
Alternative Name Immunoglobulin heavy constant mu
Background

Target Description: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Gene Symbol: IGHM

Gene ID 3507
UniProt P01871
Application Notes Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.