HLA-DQA1 antibody

Catalog No. ABIN6297784

Product Details anti-HLA-DQA1 Antibody

Target: HLA-DQA1 (Major Histocompatibility Complex, Class II, DQ alpha 1 (HLA-DQA1))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DQA1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Mouse anti-Human/Pig HLA-DQ Antibody (MHC II) [Sodium Azide Free]

Specificity: Cell surface

Purification: The T4-positive CTL clone HG-38 was used as the immunogen for the HLA-DQ antibody.

Immunogen: The T4-positive CTL clone HG-38 was used as the immunogen for the HLA-DQ antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for HLA-DQA1

Target: HLA-DQA1 (Major Histocompatibility Complex, Class II, DQ alpha 1 (HLA-DQA1))

Alternative Name: HLA class II histocompatibility antigen, DQ alpha 1 chain (HLA-DQA1 Products)

Synonyms: CD antibody, CELIAC1 antibody, DQ-A1 antibody, GSE antibody, HLA-DQA antibody, BoLA-DQA antibody, hla-dqa1 antibody, major histocompatibility complex, class II, DQ alpha 1 antibody, major histocompatibility complex, class II, DQ alpha, type 1 antibody, major histocompatibility complex, class II, DQ alpha 1 L homeolog antibody, HLA-DQA1 antibody, BOLA-DQA1 antibody, hla-dqa1.L antibody

Background:

Target Description: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.

Gene Symbol: HLA-DQA1///HLA-DQB1///HLA-DQB

UniProt: P01909, P01920

Pathways: TCR Signaling, Cancer Immune Checkpoints