Pseudouridylate Synthase 10 (PUS10) (Middle Region) antibody

Catalog No. ABIN631863

Product Details

Target: Pseudouridylate Synthase 10 (PUS10)

See all Pseudouridylate Synthase 10 (PUS10) Antibodies

Binding Specificity: Middle Region

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• AA 52-78 16
• N-Term 9
• AA 287-336 1
• Middle Region 1

Reactivity: Human

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• Human 37
• Mouse 21
• Rat 18
• Cow 2
• Dog 2
• Horse 2
• Pig 2
• Rabbit 2
• Zebrafish (Danio rerio) 2
• Bat 1
• Chicken 1
• Guinea Pig 1
• Monkey 1
• Xenopus laevis 1

Host: Rabbit

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• Rabbit 37

Clonality: Polyclonal

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• Polyclonal 37

Conjugate: Un-conjugated

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• Un-conjugated 9
• Biotin 4
• FITC 3
• HRP 3
• APC 2
• Alexa Fluor 750 2
• Alkaline Phosphatase (AP) 2
• PE 2
• Alexa Fluor 350 1
• Alexa Fluor 488 1
• Alexa Fluor 555 1
• Alexa Fluor 594 1
• Alexa Fluor 647 1
• Alexa Fluor 680 1
• Cy3 1
• Cy5 1
• Cy5.5 1
• Cy7 1

Application: Western Blotting (WB)

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• Western Blotting (WB) 25
• ELISA 17
• Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13
• Immunohistochemistry (IHC) 3
• Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3
• Flow Cytometry (FACS) 1

Specificity: PUS10 antibody was raised against the middle region of PUS10

Purification: Affinity purified

Immunogen: PUS10 antibody was raised using the middle region of PUS10 corresponding to a region with amino acids AVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFN

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

PUS10 Blocking Peptide, catalog no. 33R-1592, is also available for use as a blocking control in assays to test for specificity of this PUS10 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of PUS10 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details

Target: Pseudouridylate Synthase 10 (PUS10)

Alternative Name: PUS10 (PUS10 Antibody Abstract)

Synonyms: CCDC139, DOBI, 2810013G11Rik, 4933435A13Rik, AU014648, C77560, Ccdc139, RGD1306402, pseudouridylate synthase 10, PUS10, Pus10

Background: Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs.

Molecular Weight: 60 kDa (MW of target protein)