KCNN4 antibody (Internal Region)

Catalog No. ABIN6391378

Product Details anti-KCNN4 Antibody

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This KCNN4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: KCNN4 / KCa3.1

Sequence: ERQAVNATGH LSD

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland, Kidney and Liver. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Cerebellum lysates and in lysates of cell line NIH3T3 (calculated MW of 47.7 kDa, according to Human NP_002241.1 and Mouse NP_032459.3). An additional band of unknown identity was also consistently observe

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentratio

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KCNN4

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Alternative Name: KCNN4 (KCNN4 Products)

Background: KCNN4, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4, IK1, IKCA1, KCA4, KCa3.1, SK4, hIKCa1, hKCa4, hSK4, intermediate conductance calcium-activated potassium channel protein 1, putative erythrocyte intermediate

Gene ID: 3783, 16534, 65206

NCBI Accession: NP_002241