Methyl CpG Binding Protein 2 (MECP2) antibody

Details for Product No. ABIN6566655
Antigen
Reactivity
Human, Mouse (Murine)
213
72
52
6
6
5
4
4
3
3
3
2
1
1
Host
Rabbit
196
21
3
2
Clonality
Polyclonal
Conjugate
Un-conjugated
15
12
12
12
12
12
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
176
132
76
48
20
19
13
11
10
10
5
4
2
1
Options
Immunogen Recombinant protein of human MECP2
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name MECP2 (MECP2 Antibody Abstract)
Background

Synonyms: AUTSX 3,AUTSX3,DKFZp686A24160,Mbd 5,Mbd5,MECP 2,MeCP 2 protein,MeCP-2 protein,Mecp2,MECP2,Methyl CpG binding protein 2 (Rett syndrome),Methyl CpG binding protein 2,Methyl-CpG-binding protein 2,MRX 16,MRX 79,MRX16,MRX79,MRXS 13,MRXS13,MRXSL,PPMX,RS,RTS,RTT,WBP 10,WBP10

Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight

Observed_MW: 83kDa

Calculated_MW: 52kDa/53kDa

Gene ID 4204
UniProt P51608
Pathways Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane
Application Notes WB 1:500 - 1:1000 IHC 1:50 - 1:100
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-Methyl CpG Binding Protein 2 (MECP2) antibody (ABIN6566655) Western blot analysis of extracts of various cell lines, using MECP2 antibody.
Image no. 2 for anti-Methyl CpG Binding Protein 2 (MECP2) antibody (ABIN6566655) Immunohistochemistry of paraffin-embedded mouse brain using MECP2 antibody.
Image no. 3 for anti-Methyl CpG Binding Protein 2 (MECP2) antibody (ABIN6566655) Immunohistochemistry of paraffin-embedded human gastric cancer using MECP2 antibody.