L-Selectin antibody (APC)

Catalog No. ABIN6655166

Supplier Product No.: 200-326-n72

Product Details anti-L-Selectin Antibody (APC)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is conjugated to APC

Application: Flow Cytometry (FACS), Western Blotting (WB)

Supplier Product No.: 200-326-n72

Supplier: Rockland

Purpose: CD62L Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD62L.

Purification: Allophycocyanin conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD62L.

Sterility: Sterile filtered

Immunogen: Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with CD62L antigen.

Clone: DREG-56

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells

Western_Blot_Dilution: User Optimized

Comment:

Anti-CD62L is tested for Flow Cytometry and useful in Western Blot. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L (SELL Products)

Background:

Synonyms: L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, LAM-1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, Lymph node homing receptor, TQ1, gp90-MEL, CD62L , SELL , LNHR, LYAM1

Background: CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naive lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.

Gene Name: SELL

Gene ID: 6402

NCBI Accession: NP_000646

UniProt: P14151