IKBKG antibody

Catalog No. ABIN6655353

Supplier Product No.: 200-301-h86

Product Details anti-IKBKG Antibody

Target: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKBKG antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Supplier Product No.: 200-301-h86

Supplier: Rockland

Purpose: IKK gamma Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with IKKg from Human based on 100 % homology with the immunizing sequence.

Purification: Anti-IKKg Antibody was purified by Protein G chromatography.

Immunogen: IKK gamma Antibody was produced in mice prepared by repeated immunizations with human IKKgamma (NEMO) protein at full-length.

Clone: 46B844

Isotype: IgG1 lambda

Application Details

Application Notes:

Flow_Cytometry_Dilution: 0.1-0.5/10^6 cells

Western_Blot_Dilution: 2 μg/mL

Comment:

Anti-IKKgamma antibody is tested for use in Flow (I) and WB. Expect a band approximately 48kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IKBKG

Target: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Alternative Name: IKBKG (IKBKG Products)

Background:

Synonyms: FIP3, NEMO, NF-kappa-B essential modulator, NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma, NF-kappa-B essential modifier

Background: Anti-IKK gamma detects human IKKgamma. NF-kappaB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kappaB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKKalpha, IKKbeta, and IKKgamma (NEMO). NEMO (NF-kB Essential MOdulator) preferentially interacts with IKK2/b and is required for activation of IKK complex. Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKalpha nor IKKbeta, but complexes directly with NEMO. This suggests that NEMO may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb. Anti-IKKgamma antibody is ideal for investigators involved in NFkappaB, kinase and growth factor research.

Gene Name: IKBKG

Gene ID: 8517

NCBI Accession: NP_001093326

UniProt: Q9Y6K9

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins