PARP1 antibody (Cleavage Site)
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- Target See all PARP1 Antibodies
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
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Binding Specificity
- Cleavage Site
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PARP1 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- Supplier Product No.
- 200-301-i03
- Supplier
- Rockland
- Purpose
- PARP Antibody
- Cross-Reactivity (Details)
- A BLAST analysis was used to suggest cross-reactivity with Anti-PARP from human based on 100 % homology with the immunizing sequence.
- Purification
- Anti-PARP Antibody was purified by Protein G chromatography.
- Immunogen
- PARP Antibody was produced in mice prepared by repeated immunizations with a peptide containing amino acids near the 214/215 cleavage site of human PARP protein.
- Clone
- 194C1439
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product PARP1 Primary Antibody
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- Application Notes
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Flow_Cytometry_Dilution: 1-3 μg/test
Western_Blot_Dilution: 1-2 μg/mL
- Comment
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Anti-PARP antibody is tested for use in WB, Flow, Flow-IC, and ICC/IF. Expect a band approximately 89kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
Preservative: 0.05 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- Alternative Name
- PARP (PARP1 Products)
- Background
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Synonyms: ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, PARP-1, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1
Background: Anti-PARP antibody detects human PARP. The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks. Anti-PARP antibody is ideal for investigators involved in Apoptosis, DNA damage and Repair research.
Gene Name: PARP1
- Gene ID
- 142
- NCBI Accession
- NP_001609
- UniProt
- P09874
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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