PARP1 antibody (Cleavage Site)

Catalog No. ABIN6655433

Supplier Product No.: 200-301-i03

This anti-PARP1 antibody is a Mouse Monoclonal antibody detecting PARP1 in WB, IF and FACS. Suitable for Human.

Quick Overview for PARP1 antibody (Cleavage Site) (ABIN6655433)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 194C1439

Product Details anti-PARP1 Antibody

Binding Specificity: Cleavage Site

Supplier Product No.: 200-301-i03

Supplier: Rockland

Purpose: PARP Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-PARP from human based on 100 % homology with the immunizing sequence.

Purification: Anti-PARP Antibody was purified by Protein G chromatography.

Immunogen: PARP Antibody was produced in mice prepared by repeated immunizations with a peptide containing amino acids near the 214/215 cleavage site of human PARP protein.

Isotype: IgG2b kappa

Application Details

Application Notes:

Flow_Cytometry_Dilution: 1-3 μg/test

Western_Blot_Dilution: 1-2 μg/mL

Comment:

Anti-PARP antibody is tested for use in WB, Flow, Flow-IC, and ICC/IF. Expect a band approximately 89kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP

Background:

Synonyms: ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, PARP-1, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1

Background: Anti-PARP antibody detects human PARP. The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks. Anti-PARP antibody is ideal for investigators involved in Apoptosis, DNA damage and Repair research.

Gene Name: PARP1

Gene ID: 142

NCBI Accession: NP_001609

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location