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PARP1 antibody (Cleavage Site)

PARP1 Reactivity: Human WB, FACS, IF Host: Mouse Monoclonal 194C1439 unconjugated
Rockland
Catalog No. ABIN6655433
Supplier Product No.: 200-301-i03
  • Target See all PARP1 Antibodies
    PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
    Binding Specificity
    • 16
    • 15
    • 15
    • 10
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cleavage Site
    Reactivity
    • 217
    • 56
    • 43
    • 8
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 213
    • 21
    • 1
    Mouse
    Clonality
    • 206
    • 29
    Monoclonal
    Conjugate
    • 133
    • 16
    • 15
    • 13
    • 12
    • 12
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This PARP1 antibody is un-conjugated
    Application
    • 146
    • 118
    • 69
    • 31
    • 31
    • 25
    • 25
    • 22
    • 21
    • 17
    • 16
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
    Supplier Product No.
    200-301-i03
    Supplier
    Rockland
    Purpose
    PARP Antibody
    Cross-Reactivity (Details)
    A BLAST analysis was used to suggest cross-reactivity with Anti-PARP from human based on 100 % homology with the immunizing sequence.
    Purification
    Anti-PARP Antibody was purified by Protein G chromatography.
    Immunogen
    PARP Antibody was produced in mice prepared by repeated immunizations with a peptide containing amino acids near the 214/215 cleavage site of human PARP protein.
    Clone
    194C1439
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product PARP1 Primary Antibody
  • Application Notes

    Flow_Cytometry_Dilution: 1-3 μg/test

    Western_Blot_Dilution: 1-2 μg/mL

    Comment

    Anti-PARP antibody is tested for use in WB, Flow, Flow-IC, and ICC/IF. Expect a band approximately 89kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.05 % BSA

    Preservative: 0.05 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
    Alternative Name
    PARP (PARP1 Products)
    Background

    Synonyms: ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, PARP-1, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1

    Background: Anti-PARP antibody detects human PARP. The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks. Anti-PARP antibody is ideal for investigators involved in Apoptosis, DNA damage and Repair research.

    Gene Name: PARP1

    Gene ID
    142
    NCBI Accession
    NP_001609
    UniProt
    P09874
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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