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Carboxylesterase 1G (CES1G) antibody

CES1G Reactivity: Human ELISA, IP, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6655641
$399.00
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100 μL
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  • Target
    Carboxylesterase 1G (CES1G)
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    ELISA, Immunoprecipitation (IP), Western Blotting (WB)
    Purification
    This product was prepared from monospecific antiserum by delipidation and defibrination.   The antibody detects recombinant hCE1 in cell lysates and native Liver Carboxylesterase-1 present in human tissue or fluids in various assays (ie. IL-1 stimulated hCE1 production from fibroblasts).
    Immunogen

    Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human Liver Carboxylesterase-1.

    Immunogen Type: Recombinant Protein

  • Application Notes

    Application Note: This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase).  Specific conditions for reactivity should be optimized by the end user.   Expect a band approximately 66 kDa in size corresponding CE1 by western blotting in the appropriate cell lysate or extract.  In western blot analysis endogenous protein present in human tissue or fluids, multiple bands of CE may appear corresponding to variable amount of glycosylation on the molecule.

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Western Blot Dilution: 1:2,500 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    Carboxylesterase 1G (CES1G)
    Alternative Name
    Liver Carboxylesterase-1
    Synonyms
    ACAT, CE-1, CEH, CES2, HMSE, HMSE1, PCE-1, REH, SES1, TGH, hCE-1, Ces-1, Ces1, Ses-1, carboxylesterase 1, carboxylesterase 1G, liver carboxylesterase, similar to esterase 2, CES1, Ces1g, LOC100009551, LOC684016
    Background

    Synonyms: rabbit anti-Liver Carboxylesterase-1 Antibody, Liver carboxylesterase 1, Acyl coenzyme A:cholesterol acyltransferase, ACAT, Monocyte/macrophage serine esterase, HMSE, Serine esterase 1, Brain carboxylesterase hBr1, Triacylglycerol hydrolase, Egasyn, Retinyl ester hydrolase, REH

    Background: Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism). Drug half-life and bioavailability may be modulated by selective inhibition of these proteins. CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.

    Gene Name: CES1

    Gene ID
    1066, 68508965
    UniProt
    P23141
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