SMC1A antibody (AA 951-962)

Catalog No. ABIN6655981

Product Details anti-SMC1A Antibody

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Binding Specificity: AA 951-962

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine)

Purification: This Protein G Purified Mab antibody is directed against human SMC1 and is useful in determining its presence in various assays. This monoclonal anti-SMC1 antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.  Minimal reactivity is observed against the non-phosphorylated epitope.  Reactivity is observed against human and mouse SMC1.  Cross reactivity with SMC1 from other eukaryotic sources has not been tested.

Immunogen:

Immunogen: This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse.

Immunogen Type: Peptide

Clone: 5D11-G5

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Protein G Purified Mab anti-SMC1 was tested by ELISA, immunohistochemistry and western blotting against native protein. The antibody reacts with SMC1 from irradiated human and mouse cells.  A 160 kDa band corresponding to phosphorylated human SMC1 is noted in gamma irradiated human and mouse lysates.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:100 - 1:2,000

IF Microscopy Dilution: 2.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-SMC1A antibody (ABIN6655981)

Callén, Jankovic, Wong, Zha, Chen, Difilippantonio, Di Virgilio, Heidkamp, Alt, Nussenzweig, Nussenzweig: "Essential role for DNA-PKcs in DNA double-strand break repair and apoptosis in ATM-deficient lymphocytes." in: Molecular cell, Vol. 34, Issue 3, pp. 285-97, (2009) (PubMed).

Pusapati, Rounbehler, Hong, Powers, Yan, Kiguchi, McArthur, Wong, Johnson: "ATM promotes apoptosis and suppresses tumorigenesis in response to Myc." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1446-51, (2006) (PubMed).

Kitagawa, Bakkenist, McKinnon, Kastan: "Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway." in: Genes & development, Vol. 18, Issue 12, pp. 1423-38, (2004) (PubMed).

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1 (SMC1A Products)

Synonyms: CDLS2 antibody, DXS423E antibody, SB1.8 antibody, SMC1 antibody, SMC1L1 antibody, SMC1alpha antibody, SMCB antibody, SMC-1A antibody, NV15570 antibody, 5830426I24Rik antibody, Sb1.8 antibody, Smc1 antibody, Smc1alpha antibody, Smc1l1 antibody, Smcb antibody, mKIAA0178 antibody, smc1 antibody, smc1a antibody, smc1l1 antibody, xSMC1 antibody, smc1aa antibody, structural maintenance of chromosomes 1A antibody, structural maintenance of chromosomes 1A b antibody, structural maintenance of chromosomes 1A L homeolog antibody, SMC1A antibody, smc1a antibody, Smc1a antibody, smc1ab antibody, smc1a.L antibody

Background:

Synonyms: mouse anti-SMC1 pS957 antibody, Structural maintenance of chromosomes protein 1B antibody, SMC1beta protein antibody, SMC1B antibody, SMC1L2 antibody

Background: Structural maintenance of chromosomes (SMC) proteins play important roles in sister chromatid cohesion, chromosome condensation, sex-chromosome dosage compensation, and DNA recombination and repair (DNA damage). Protein complexes containing heterodimers of the SMC1 and SMC3 proteins have been implicated specifically in both sister chromatid cohesion and DNA recombination.  ATM, a protein kinase belonging to the phosphatidylinositol 3-kinase family that regulates cell cycle checkpoints and DNA recombination and repair, phosphorylates SMC1 protein after ionizing irradiation. ATM protein kinase phosphorylates SMC1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint.  Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM protein kinase substrates NBS1 and BRCA1. These same sites in SMC1 are phosphorylated after treatment with UV irradiation or hydroxyurea in an ATM-independent manner, thus demonstrating that another kinase must be involved in responses to these cellular stresses. Yeast containing hypomorphic mutations in SMC1 and human cells overexpressing SMC1 mutated at both of these phosphorylation sites exhibit decreased survival following ionizing irradiation. These results demonstrate that SMC1 participates in cellular responses to DNA damage and link SMC1 to the ATM protein kinase signal transduction pathway.

Gene Name: SMC1B

Gene ID: 27127

UniProt: Q8NDV3

Pathways: Stem Cell Maintenance