PINK1 antibody (AA 112-496)

Catalog No. ABIN6656304

Supplier Product No.: 200-301-w64

Product Details anti-PINK1 Antibody

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Binding Specificity: AA 112-496

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PINK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-w64

Supplier: Rockland

Purpose: PINK1 Antibody

Cross-Reactivity (Details): BLAST analysis suggests that it is 82 % identical to rat, 81 % identical to mouse and >30 % identical to DMPK.

Purification: Anti-PINK1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-PINK1 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 112-496 of human PINK1.

Clone: S4-15

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Other: Immunocytochemistry/Immunofluorescence 1:100

Comment:

Anti-PINK1 Antibody has been tested in WB, IHC, and ICC/IF. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PINK1

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Alternative Name: PINK1 (PINK1 Products)

Background:

Synonyms: Parkinson disease (autosomal recessive) 6, EC 2.7.11.1, PARK6, serine/threonine-protein kinase PINK1 mitochondrial, PTEN-induced putative kinase protein 1, protein kinase BRPK, BRPK, FLJ27236, PTEN induced putative kinase 1, Phosphatase and Tensin Homolog

Background: PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports, PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction. Areas of interest and use for researchers include Neuroscience, mitochondrial function, and CDK-mediated phosphorylation pathways.

Gene Name: PINK1

Gene ID: 65018

NCBI Accession: NP_115785

Pathways: Autophagy